Summary
The WCM CLC Flow Cytometry Core Facility offers state-of-the-art fluorescence activated cell sorting (FACS) instruments and services, and expertise in their applications, to the Weill Cornell Medicine (WCM) community and to outside investigators. The facility also provides consultation on project design and data analysis, and offers educational workshops, seminars and training.
- Partnering for quality flow cytometry to maximize scientific impact.
- Providing cutting edge instrumentation maintained and quality controlled to the highest standard.
- Serving the scientific community through innovative training and education.
- Focused on reliable, valuable, and quality services.
- Striving for continual improvement for our partners and staff.
Overview
History: The Flow Cytometry Core Facility was established in 2012 by the Department of Pathology and became part of the institutional WCM Core Laboratories Center (CLC) in 2015. The facility expanded into a multi-institutional core in 2016 as part of a collaboration between Weill Cornell Medicine (WCM) and the Hospital for Special Surgery (HSS).
Location: The core is located in the sub-basement level of the “E” building in room E-0033, 1300 York Avenue, New York, NY.
WCM Core Laboratories Center (CLC): The WCM CLC was established in 2015. The CLC includes core facilities that offer resources and services in genomics and epigenomics, proteomics and metabolomics, flow cytometry, imaging (e.g., optical and electron microscopy, high content screening, MRI, PET/CT, and ultrasound), synthetic and analytical chemistry, and nuclear magnetic resonance (NMR).
Open to all: The resources and services of the core facility are open to all investigators at Weill Cornell Medicine, Cornell University and Cornell-affiliated institutions. The facility also provides services to external investigators at both academic institutions and commercial enterprises.
Resources
Cell sorters:
BD S6 (spectral), 5-laser (355, 405, 488, 561, 640), 50 spectral bands, capable of 6-way sorting
2 - BD S6 (conventional), 5-laser (355, 405, 488, 561, 640), 30 detectors, capable of 6-way sorting
BD Influx sorter, 6-laser (355, 405, 445, 488, 561, 640), 22 detectors, capable of 6-way sorting
Sony MA900 sorter, 4-laser (405, 488, 561, 630), 12 detectors, capable of 4-way sorting
Analytical cytometers:
BD FACSymphony S6, 5-laser (355, 305, 488, 561, 640), 29 detectors
BD Fortessa analyzer, 5-laser (355, 405, 488, 561, 640) 20 detectors
BD FACSCelesta analyzer, 3-laser (405, 488, 561) 14 detectors
Data analysis:
WCM Library partnership for FlowJo and FCS Express site licenses
Data analysis help by appointment – contact the core!
Services
Consultation
Core staff is available to consult on all things Flow Cytometry; experiment design, choosing fluorochromes, sample preparation etc.
Operator Assisted Sorting
Cell sorting service provided by highly skilled staff to users on an hourly basis on BD instruments. Available Monday - Thursday 10 a.m. to 6 p.m., Friday 10 a.m. to 5p.m..
Self-Service Analyzers
All analytical cytometers available 24/7 to flow core trained or certified users.
Self-Service Sorting
Sony MA900 or BD S6 sorter after required training.
Seminars, Workshops, and Training
Monthly Introduction to Flow Cytometry Class with hands-on analyzer session. Designed for new or novice users but often helpful as a brush-up for more seasoned researchers. Note: this course is a prerequisite for self-service analyzer use and self-service sorter training.
Self-service sorter training -small, focused group sessions and individual sessions
Sponsor and host a variety of informative and educational seminars throughout the year. Watch out for center wide email announcements.
Contact Information
Jason McCormick
Core Director, Flow Cytometry
646-926-7611
jam2073@med.cornell.edu
Tomas Baumgartner
Core Director, Flow Cytometry
646-926-7611
tob2008@med.cornell.edu
More Information
For more information about services and pricing, to register as a user of the core, to submit samples, to schedule time on the instruments, and/or to arrange for training, please go to the WCM iLab portal at http://wcmc.corefacilities.org. Please select the Flow Cytometry Core Facility once you are on the iLab page.
For additional information on services, consultation, and pricing please contact one of the managers via email or at their on-site facility in E-0033.