The Microbiome Core specializes in bacterial analysis and provides institutional sample-to-data processing services, and accepts a wide variety of sample types (mouse/rat feces, human feces, human/animal swabs, pelleted cells, or just extracted DNA). With a streamlined and highly efficient process, the Microbiome Core can deliver data to customers anywhere in the world in as little as three weeks. Clients have ranged from local investigators from Weill Cornell Medicine, Memorial Sloan Kettering, and The Rockefeller University, to external users from academic institutions and commercial enterprises.
Core Technologies/Services
- 16S Sequencing: DNA extractions are performed utilizing the Promega MaxPrep & Maxwell RSC 48 system. In tandem, they provide quick DNA isolation of up to 96 samples per-day with minimal hands-on effort.
- Shotgun Metagenomic Sequencing: Like 16S processing, the Core performs DNA extractions utilizing the Promega MaxPrep & Maxwell RSC 48 system.
Equipment
- Promega MaxPrep
- Promega Maxwell RSC 48
- Promega Glomax Discover
- Perkin Elmer Zephyr G3 NGS Workstation
- Perkin Elmer GX Touch HT Nucleic Acid Analyzer
- Perkin Elmer Zephyr G3 NGS Workstation
- MiSeq (16S Sequencing; paired-end 250, 7pM loading concentration, 15% PhiX spike-in)
- HiSeq-X/NextSeq500/NovaSeq6000 (Shotgun Metagenomic Sequencing; 10pM loading concentration, 0%PhiX spike-in