The Genomics Resources Core Facility (GRCF) at WCM, established in 2000, plays a pivotal role in advancing genomics research both internally and externally. It offers a comprehensive range of services from experimental design and sample preparation to instrumentation, data analysis, interpretation, and validation.
At the core of its operations are cutting-edge sequencing technologies and a team of well-trained staff, enabling researchers to leverage state-of-the-art technologies effectively. These resources have not only supported but significantly contributed to numerous publications and successful grant applications within the research community.
The mission of the GRCF is clear: to provide access to high-quality genomics services at affordable costs, thereby fostering innovation and collaboration across the research landscape. By maintaining a commitment to excellence in service delivery and cost-effectiveness, the facility continues to play a critical role in advancing genomic research and its applications.
Instrumentation
1. Illumina NGS sequencers (short-read): NovaSeq X Plus, NextSeq2000, NextSeq500, MiSeq.
2. PacBio Revio sequencer (long-read).
3. 10X Genomics single-cell sequencing system: Chromium X, Chromium controller.
4. Automation platforms: SPT labtech Firefly system, Agilent Bravo B, Integra Miro Canvas.
5. gDNA shearing system: Covaris Focused Ultrasonicate R230, Diagenode Megaruptor 3.
6. Sample quality check system: Agilent TapeStation, Bioanalyzer, Femto Pulse.
7. Sample quantity check system: Nanodrop UV Spectrophotometer, Qubit Fluorometer.
Services
1. Consultation: to provide options and recommend the optimal experimental strategy.
2. Sample quality control: including quality and quantity measurement.
3. Library construction (manual or robot):
- For DNA: whole genome sequencing, whole exome sequencing, PCR amplicon, Metagenomics, ChIP-Seq, targeted sequencing (panel), sequencing for CRISPR screening.
- For RNA: whole transcriptome sequencing, capture-based RNA-Seq, miRNA-Seq.
4. Single-cell sample preparation and sequencing:
- Single-cell 3' Library RNA-Seq Assays (GEM-X v4 kit)
- Single-cell 5' Library RNA-Seq Assays (GEM-X v3 kit)
- Single Cell Multiome ATAC + Gene Expression
- Single Cell ATAC-Seq
- Cell Multiplexing Library Construction (Additional to Single-cell 3' Lib prep)
- Single Cell Human TCR / BCR Enrichment (Additional to Single-cell 5' Lib prep)
- CRISPR Screening Library Construction (Additional to Single-cell 3' Lib prep)
5. Sequencing:
- Illumina sequencing systems – NovaSeq X Plus, NextSeq2000, NextSeq500, MiSeq
- PacBio sequencing system - Revio
6. Spatial Nanostring GeoMx: NGS Readout Library Prep.
7. Genomics data storage, management, and bioinformatics support: including primary, secondary and tertiary data analysis, data storage and management.
8. Other support: offering support letter and writing genomics part for PIs’ grant and manuscript preparations.