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WCM Supply Center

WCM Supply Centers

        Product List at Belfer Building

Product Name                    

Size   

Cat. No.

Cell Culture & Transfection Reagents:

Antibiotics & Antimycotics
Penicillin-Streptomycin (10,000 U/mL)100 mL15140122
Basal Media
DMEM/F-12, GlutaMAX™ supplement500 mL10565018            
KnockOut™ DMEM500 mL10829018
DMEM, low glucose, pyruvate, no glutamine, no phenol red1 L                11054001           
DMEM/F-12500 mL11320033
DMEM/F-12, HEPES500 mL11330032
RPMI 1640 Medium500 mL11875093
RPMI 1640 Medium, no glucose500 mL11879020
DMEM, high glucose500 mL11965092
DMEM, high glucose, pyruvate500 mL11995065
IMDM500 mL12440053
Advanced DMEM/F-12500 mL12634010
RPMI 1640 Medium, HEPES500 mL22400097
Opti-MEM™ I Reduced Serum Medium500 mL31985070
MEM, no glutamine, no phenol red500 mL51200038
RPMI 1640 Medium (ATCC modification)500 mLA1049101
Cell Culture Supplements
MEM Non-Essential Amino Acids Solution (100X)                           100 mL           11140050              
Sodium Pyruvate (100 mM)100 mL11360070
B-27™ Supplement (50X), minus vitamin A10 mL12587010
N-2 Supplement (100X)5 mL17502048
B-27™ Supplement (50X), serum free10 mL17504044
L-Glutamine (200 mM)100 mL25030081
Insulin-Transferrin-Selenium-Ethanolamine (ITS -X) (100X)10 mL51500056
Glucose Solution50 mLA2494001
Cell Dissociation Reagents
Trypsin-EDTA (0.25%), phenol red       100 mL25200056
Trypsin-EDTA (0.05%), phenol red100 mL25300054
Cryopreservation Media
Recovery™ Cell Culture Freezing Medium50 mL    12648010
Microbiological Media
LB Broth500 mL    10855021
Serum
Fetal Bovine Serum, qualified, heat inactivated, USDA-approved regions500 mL             10438026                
Fetal Bovine Serum, qualified, United States500 mL26140079
Fetal Bovine Serum, qualified, One Shot™ format, United States50 mLA3160501
Serum-Free & Specialty Media
Neurobasal™-A Medium                       500 mL10888022
Neurobasal™ Medium500 mL21103049
 

Gels, Fractionation Strips, & Membranes

Pre-Cast Protein Gels
NuPAGE™ 4-12% Bis-Tris Protein Gels, 1.5 mm, 10-well10 gels (1 box)NP0335BOX           
Bolt™ 4-12% Bis-Tris Plus Gels, 10-well10 gels (1 box)NW04120BOX
Novex™ 10% Tris-Glycine Mini Gels, WedgeWell™ format, 12-well10 gels (1 box)XP00102BOX

Kits and Assays

cDNA Synthesis, Library, & Cloning Kits
High-Capacity RNA-to-cDNA™ Kit                                      50 reactions4387406            
TaqMan™ Reverse Transcription Reagents200 reactionsN8080234
Real-Time PCR and Endpoint RT-PCR Kits
PowerUp™ SYBR™ Green Master Mix                                1 mLA25741        
PowerUp™ SYBR™ Green Master Mix5 mLA25742
TaqMan™ Gene Expression Master Mix1 x 5 mL4369016
Fast SYBR™ Green Master Mix1 x 5 mL4385612
TaqMan™ Universal Master Mix II, no UNG1 x 5 mL4440040
TaqMan™ Fast Advanced Master Mix1 x 5 mL4444557

 

Lab Reagents and Chemicals

Flow Cytometer Buffers & Reagents
Flow Cytometer Buffers & Reagents100 tests01-2222-42
General Buffers for Life Science
HEPES (1 M)                                            100 mL  15630080

 

Labeling & Detection Products

Enzyme Substrates
Pierce™ ECL Plus Western Blotting Substrate100 mL         32132             
SuperSignal™ West Femto Maximum Sensitivity Substrate100 mL34095

Oligos, Primers, Probes & Nucleotides

DNA Primers & Oligos
  Random Hexamers (50 μM)                   5 nmol   N8080127
Nucleotides
dNTP Set 100 mM Solutions                                    4 x 0.25 mLR0181           

Proteins, Enzymes, & Peptides

Enzyme Inhibitors
SUPERase• In™ RNase Inhibitor (20 U/μL)                2,500 units AM2694        
RNaseOUT™ Recombinant Ribonuclease Inhibitor5,000 units10777019
PCR & Cloning Enzymes
Phusion Hot Start II High-Fidelity PCR Master Mix100 reactions F565S               
DreamTaq PCR Master Mix (2X)200 reactionsK1071
Proteinase K, recombinant, PCR grade5 x 1.0 mLEO0492
SuperScript™ IV VILO™ Master Mix with ezDNase™ Enzyme50 reactions11766050
Platinum™ SuperFi™ Green PCR Master Mix100 reactions12359010
Platinum™ II Hot-Start PCR Master Mix (2X)200 reactions14000013
T4 DNA Ligase (1 U/μL)100 units15224017
DNase I, Amplification Grade100 units18068015
SuperScript™ IV Reverse Transcriptase10,000 units18090050

Standards, Ladders & Controls

DNA⁄RNA Ladders
GeneRuler 100 bp Plus DNA Ladder, ready-to-use                    5 x 50 μgSM0324               
GeneRuler 1 kb Plus DNA Ladder, ready-to-use5 x 50 μgSM1333
E-Gel™ 1 Kb Plus Express DNA Ladder100 applications10488091
Protein Standards & Ladders
PageRuler™ Plus Prestained Protein Ladder, 10 to 250 kDa  2 x 250 μL       26619               
MagicMark™ XP Western Protein Standard250 μLLC5602

       Product List at LC Building Basement

Product Name                    

Size   

Cat. No.

Cell Culture & Transfection Reagents:

Antibiotics & Antimycotics
Penicillin-Streptomycin (10,000 U/mL)100 mL15140122
Basal Media
DMEM/F-12, HEPES500 mL11330032       
IMDM500 mL12440053
Advanced DMEM/F-121 L                12634010
Cell Culture Supplements
MEM Non-Essential Amino Acids Solution (100X)100 mL           11140050        
B-27™ Supplement (50X), minus vitamin A10 mL12587010
B-27™ Supplement (50X), serum free10 mL17504044
L-Glutamine (200 mM)100 mL25030081
GlutaMAX™ Supplement100 mL35050061
Cell Dissociation Reagents
Trypsin-EDTA (0.25%), phenol red100 mL25200056
Trypsin-EDTA (0.25%), phenol red100 mL25300054
Cryopreservation Media
Recovery™ Cell Culture Freezing Medium50 mL    12648010
Serum-Free & Specialty Media
Neurobasal™-A Medium                       500 mL10888022
Neurobasal™ Medium500 mL21103049
Transfection Reagents
Lipofectamine™ 2000 Transfection Reagent                   1.5 mL11668019
Lipofectamine™ 3000 Transfection Reagent0.75 mLL3000008
Lipofectamine™ 3000 Transfection Reagent1.5 mLL3000015
 

Gels, Fractionation Strips, & Membranes

Pre-Cast Protein Gels
NuPAGE™ 4-12% Bis-Tris Protein Gels, 1.5 mm, 10-well10 gels (1 box)NP0335BOX
NuPAGE™ 4-12% Bis-Tris Protein Gels, 1.5 mm, 15-well10 gels (1 box)NP0336BOX

Kits and Assays

cDNA Synthesis, Library, & Cloning Kits
High-Capacity RNA-to-cDNA™ Kit                                      50 reactions4387406            
DNA⁄RNA Purification Kits & Reagents
TRIzol™ Reagent                              200 mL15596018        
Real-Time PCR and Endpoint RT-PCR Kits
PowerUp™ SYBR™ Green Master Mix                                1 mLA25741        
SYBR™ Green PCR Master Mix1 x 50 mL4367660
Power SYBR™ Green PCR Master Mix  
TaqMan™ Gene Expression Master Mix1 x 5 mL4369016
Fast SYBR™ Green Master Mix1 x 5 mL4385612
TaqMan™ Universal Master Mix II, no UNG1 x 5 mL 
TaqMan™ Fast Advanced Master Mix1 x 5 mL4444557

 

Lab Reagents and Chemicals

Cell Lysis & Organelle Isolation Reagents
ACK Lysing Buffer100 mLA1049201

Electrophoresis Reagents & Buffers
NuPAGE™ MOPS SDS Running Buffer (20X)                  500 mL NP0001
General Buffers for Life Science
HEPES (1 M)           500 mL15630080

 

Labeling & Detection Products

Gel & Membrane Stains
SYBR™ Safe DNA Gel Stain400 μL     S33102

Oligos, Primers, Probes & Nucleotides

DNA Primers & Oligos
Random Hexamers (50 μM)        5 nmol N8080127

Proteins, Enzymes, & Peptides

Enzyme Inhibitors
RNaseOUT™ Recombinant Ribonuclease Inhibitor5,000 units10777019        

Standards, Ladders & Controls

Protein Standards & Ladders
PageRuler™ Plus Prestained Protein Ladder, 10 to 250 kDa2 x 250 μL 26619             

 

FAQs:

1 - How do I get set up to access the machines? 

  • Select the Request Access button (on one of the machines), complete the information requested and submit
  • You will receive an email message from Customer Service with your username and password within 48 hours

2 - To sign in without an access badge:

  • Select Access without a badge and enter your username and password

3 - How do I Get an Access Badge?

4 - How do I Activate my Supply Center badge?

    • Sign in to thermofisher.com
    • Go to Supply Center Settings under Your Account
    • Enter your unique access badge number
    • You can now use your badge to access the Supply Center

5 - Which payment method should I use to make my purchases?

  • We strongly recommend using your current LOC for Life Technologies.
    • You can also have your badge directly linked to your Fund/WBS account number.
    • If you don’t have a LOC set up for Life Technologies or you anticipate using the Supply Center on a regular basis, you should set up a LOC for use with the Supply Center.
    • You can make modifications to your payment/cost center under Your Account at thermofisher.com

6 - Can I use my PCard with the Supply Center?

  • We are only able to link your LOC/Fund/WBS number to your access badge

7 - How do I make a Purchase at the Supply Center?

Swipe your Access Badge to sign in or sign in without a badge by selecting Access without a Badge and enter your username and password.

  1. Confirm payment and cost center information; select Access (Note: you can make modifications to your payment/cost center values under your Account at thermofisher.com).
  2. The Supply Center door will unlock; open the door and remove the product(s) you need.
  3. Close the Supply Center door, select Done on the screen. An email order confirmation with details of your order will be sent to you.

8 - What if I selected an item in error?

9 - What if the product I need to order is not at the Supply Center?

  • You will have to order that product directly through SciQuest

10 - What if there is not enough of the product I need to order?

  • You will have to order the total amount of the product you need directly through SciQuest

 

What if I have further questions?

To find out more regarding product stock and make product requests please contact the Supply Center 800-955-6288 or email: supplycenters@thermofisher.com

Purchasing questions contact Priscilla Ledesma prl4003@med.cornell.edu

Life Technologies Representative please contact Smiti Khanna, Smiti.Khanna@thermofisher.com