Product List at Belfer Building
Product Name | Size | Cat. No. |
Cell Culture & Transfection Reagents:
Antibiotics & Antimycotics
Penicillin-Streptomycin (10,000 U/mL) | 100 mL | 15140122 |
Basal Media
DMEM/F-12, GlutaMAX™ supplement | 500 mL | 10565018 |
KnockOut™ DMEM | 500 mL | 10829018 |
DMEM, low glucose, pyruvate, no glutamine, no phenol red | 1 L | 11054001 |
DMEM/F-12 | 500 mL | 11320033 |
DMEM/F-12, HEPES | 500 mL | 11330032 |
RPMI 1640 Medium | 500 mL | 11875093 |
RPMI 1640 Medium, no glucose | 500 mL | 11879020 |
DMEM, high glucose | 500 mL | 11965092 |
DMEM, high glucose, pyruvate | 500 mL | 11995065 |
IMDM | 500 mL | 12440053 |
Advanced DMEM/F-12 | 500 mL | 12634010 |
RPMI 1640 Medium, HEPES | 500 mL | 22400097 |
Opti-MEM™ I Reduced Serum Medium | 500 mL | 31985070 |
MEM, no glutamine, no phenol red | 500 mL | 51200038 |
RPMI 1640 Medium (ATCC modification) | 500 mL | A1049101 |
Cell Culture Supplements
MEM Non-Essential Amino Acids Solution (100X) | 100 mL | 11140050 |
Sodium Pyruvate (100 mM) | 100 mL | 11360070 |
B-27™ Supplement (50X), minus vitamin A | 10 mL | 12587010 |
N-2 Supplement (100X) | 5 mL | 17502048 |
B-27™ Supplement (50X), serum free | 10 mL | 17504044 |
L-Glutamine (200 mM) | 100 mL | 25030081 |
Insulin-Transferrin-Selenium-Ethanolamine (ITS -X) (100X) | 10 mL | 51500056 |
Glucose Solution | 50 mL | A2494001 |
Cell Dissociation Reagents
Trypsin-EDTA (0.25%), phenol red | 100 mL | 25200056 |
Trypsin-EDTA (0.05%), phenol red | 100 mL | 25300054 |
Cryopreservation Media
Recovery™ Cell Culture Freezing Medium | 50 mL | 12648010 |
Microbiological Media
Serum
Fetal Bovine Serum, qualified, heat inactivated, USDA-approved regions | 500 mL | 10438026 |
Fetal Bovine Serum, qualified, United States | 500 mL | 26140079 |
Fetal Bovine Serum, qualified, One Shot™ format, United States | 50 mL | A3160501 |
Serum-Free & Specialty Media
Neurobasal™-A Medium | 500 mL | 10888022 |
Neurobasal™ Medium | 500 mL | 21103049 |
Gels, Fractionation Strips, & Membranes
Pre-Cast Protein Gels
NuPAGE™ 4-12% Bis-Tris Protein Gels, 1.5 mm, 10-well | 10 gels (1 box) | NP0335BOX |
Bolt™ 4-12% Bis-Tris Plus Gels, 10-well | 10 gels (1 box) | NW04120BOX |
Novex™ 10% Tris-Glycine Mini Gels, WedgeWell™ format, 12-well | 10 gels (1 box) | XP00102BOX |
Kits and Assays
cDNA Synthesis, Library, & Cloning Kits
High-Capacity RNA-to-cDNA™ Kit | 50 reactions | 4387406 |
TaqMan™ Reverse Transcription Reagents | 200 reactions | N8080234 |
Real-Time PCR and Endpoint RT-PCR Kits
PowerUp™ SYBR™ Green Master Mix | 1 mL | A25741 |
PowerUp™ SYBR™ Green Master Mix | 5 mL | A25742 |
TaqMan™ Gene Expression Master Mix | 1 x 5 mL | 4369016 |
Fast SYBR™ Green Master Mix | 1 x 5 mL | 4385612 |
TaqMan™ Universal Master Mix II, no UNG | 1 x 5 mL | 4440040 |
TaqMan™ Fast Advanced Master Mix | 1 x 5 mL | 4444557 |
Lab Reagents and Chemicals
Flow Cytometer Buffers & Reagents
Flow Cytometer Buffers & Reagents | 100 tests | 01-2222-42 |
General Buffers for Life Science
HEPES (1 M) | 100 mL | 15630080 |
Labeling & Detection Products
Enzyme Substrates
Pierce™ ECL Plus Western Blotting Substrate | 100 mL | 32132 |
SuperSignal™ West Femto Maximum Sensitivity Substrate | 100 mL | 34095 |
Oligos, Primers, Probes & Nucleotides
DNA Primers & Oligos
Random Hexamers (50 μM) | 5 nmol | N8080127 |
Nucleotides
dNTP Set 100 mM Solutions | 4 x 0.25 mL | R0181 |
Proteins, Enzymes, & Peptides
Enzyme Inhibitors
SUPERase• In™ RNase Inhibitor (20 U/μL) | 2,500 units | AM2694 |
RNaseOUT™ Recombinant Ribonuclease Inhibitor | 5,000 units | 10777019 |
PCR & Cloning Enzymes
Phusion Hot Start II High-Fidelity PCR Master Mix | 100 reactions | F565S |
DreamTaq PCR Master Mix (2X) | 200 reactions | K1071 |
Proteinase K, recombinant, PCR grade | 5 x 1.0 mL | EO0492 |
SuperScript™ IV VILO™ Master Mix with ezDNase™ Enzyme | 50 reactions | 11766050 |
Platinum™ SuperFi™ Green PCR Master Mix | 100 reactions | 12359010 |
Platinum™ II Hot-Start PCR Master Mix (2X) | 200 reactions | 14000013 |
T4 DNA Ligase (1 U/μL) | 100 units | 15224017 |
DNase I, Amplification Grade | 100 units | 18068015 |
SuperScript™ IV Reverse Transcriptase | 10,000 units | 18090050 |
Standards, Ladders & Controls
DNA⁄RNA Ladders
GeneRuler 100 bp Plus DNA Ladder, ready-to-use | 5 x 50 μg | SM0324 |
GeneRuler 1 kb Plus DNA Ladder, ready-to-use | 5 x 50 μg | SM1333 |
E-Gel™ 1 Kb Plus Express DNA Ladder | 100 applications | 10488091 |
Protein Standards & Ladders
PageRuler™ Plus Prestained Protein Ladder, 10 to 250 kDa | 2 x 250 μL | 26619 |
MagicMark™ XP Western Protein Standard | 250 μL | LC5602 |
Product List at LC Building Basement
Product Name | Size | Cat. No. |
Cell Culture & Transfection Reagents:
Antibiotics & Antimycotics
Penicillin-Streptomycin (10,000 U/mL) | 100 mL | 15140122 |
Basal Media
DMEM/F-12, HEPES | 500 mL | 11330032 |
IMDM | 500 mL | 12440053 |
Advanced DMEM/F-12 | 1 L | 12634010 |
Cell Culture Supplements
MEM Non-Essential Amino Acids Solution (100X) | 100 mL | 11140050 |
B-27™ Supplement (50X), minus vitamin A | 10 mL | 12587010 |
B-27™ Supplement (50X), serum free | 10 mL | 17504044 |
L-Glutamine (200 mM) | 100 mL | 25030081 |
GlutaMAX™ Supplement | 100 mL | 35050061 |
Cell Dissociation Reagents
Trypsin-EDTA (0.25%), phenol red | 100 mL | 25200056 |
Trypsin-EDTA (0.25%), phenol red | 100 mL | 25300054 |
Cryopreservation Media
Recovery™ Cell Culture Freezing Medium | 50 mL | 12648010 |
Serum-Free & Specialty Media
Neurobasal™-A Medium | 500 mL | 10888022 |
Neurobasal™ Medium | 500 mL | 21103049 |
Transfection Reagents
Lipofectamine™ 2000 Transfection Reagent | 1.5 mL | 11668019 |
Lipofectamine™ 3000 Transfection Reagent | 0.75 mL | L3000008 |
Lipofectamine™ 3000 Transfection Reagent | 1.5 mL | L3000015 |
Gels, Fractionation Strips, & Membranes
Pre-Cast Protein Gels
NuPAGE™ 4-12% Bis-Tris Protein Gels, 1.5 mm, 10-well | 10 gels (1 box) | NP0335BOX |
NuPAGE™ 4-12% Bis-Tris Protein Gels, 1.5 mm, 15-well | 10 gels (1 box) | NP0336BOX |
Kits and Assays
cDNA Synthesis, Library, & Cloning Kits
High-Capacity RNA-to-cDNA™ Kit | 50 reactions | 4387406 |
DNA⁄RNA Purification Kits & Reagents
TRIzol™ Reagent | 200 mL | 15596018 |
Real-Time PCR and Endpoint RT-PCR Kits
PowerUp™ SYBR™ Green Master Mix | 1 mL | A25741 |
SYBR™ Green PCR Master Mix | 1 x 50 mL | 4367660 |
Power SYBR™ Green PCR Master Mix | | |
TaqMan™ Gene Expression Master Mix | 1 x 5 mL | 4369016 |
Fast SYBR™ Green Master Mix | 1 x 5 mL | 4385612 |
TaqMan™ Universal Master Mix II, no UNG | 1 x 5 mL | |
TaqMan™ Fast Advanced Master Mix | 1 x 5 mL | 4444557 |
Lab Reagents and Chemicals
Cell Lysis & Organelle Isolation Reagents
ACK Lysing Buffer | 100 mL | A1049201 |
Electrophoresis Reagents & Buffers
NuPAGE™ MOPS SDS Running Buffer (20X) | 500 mL | NP0001 |
General Buffers for Life Science
HEPES (1 M) | 500 mL | 15630080 |
Labeling & Detection Products
Gel & Membrane Stains
SYBR™ Safe DNA Gel Stain | 400 μL | S33102 |
Oligos, Primers, Probes & Nucleotides
DNA Primers & Oligos
Random Hexamers (50 μM) | 5 nmol | N8080127 |
Proteins, Enzymes, & Peptides
Enzyme Inhibitors
RNaseOUT™ Recombinant Ribonuclease Inhibitor | 5,000 units | 10777019 |
Standards, Ladders & Controls
Protein Standards & Ladders
PageRuler™ Plus Prestained Protein Ladder, 10 to 250 kDa | 2 x 250 μL | 26619 |
FAQs:
1 - How do I get set up to access the machines?
- Select the Request Access button (on one of the machines), complete the information requested and submit
- You will receive an email message from Customer Service with your username and password within 48 hours
2 - To sign in without an access badge:
- Select Access without a badge and enter your username and password
3 - How do I Get an Access Badge?
4 - How do I Activate my Supply Center badge?
- Sign in to thermofisher.com
- Go to Supply Center Settings under Your Account
- Enter your unique access badge number
- You can now use your badge to access the Supply Center
5 - Which payment method should I use to make my purchases?
- We strongly recommend using your current LOC for Life Technologies.
- You can also have your badge directly linked to your Fund/WBS account number.
- If you don’t have a LOC set up for Life Technologies or you anticipate using the Supply Center on a regular basis, you should set up a LOC for use with the Supply Center.
- You can make modifications to your payment/cost center under Your Account at thermofisher.com
6 - Can I use my PCard with the Supply Center?
- We are only able to link your LOC/Fund/WBS number to your access badge
7 - How do I make a Purchase at the Supply Center?
Swipe your Access Badge to sign in or sign in without a badge by selecting Access without a Badge and enter your username and password.
- Confirm payment and cost center information; select Access (Note: you can make modifications to your payment/cost center values under your Account at thermofisher.com).
- The Supply Center door will unlock; open the door and remove the product(s) you need.
- Close the Supply Center door, select Done on the screen. An email order confirmation with details of your order will be sent to you.
8 - What if I selected an item in error?
9 - What if the product I need to order is not at the Supply Center?
- You will have to order that product directly through Guided Buying.
10 - What if there is not enough of the product I need to order?
- You will have to order the total amount of the product you need directly through Guided Buying.
What if I have further questions?
To find out more regarding product stock and make product requests please contact the Supply Center 800-955-6288 or email: supplycenters@thermofisher.com
Purchasing questions contact Priscilla Ledesma prl4003@med.cornell.edu
Life Technologies Representative please contact Smiti Khanna, Smiti.Khanna@thermofisher.com