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         Common Stock List 

                        

Product Name                    

Size   

Cat. No.

Cell Culture & Transfection Reagents:

Antibiotics & Antimycotics
Penicillin-Streptomycin (5,000 U/mL)100 mL15070063
Penicillin-Streptomycin (10,000 U/mL)100 mL15140122
Basal Media
DMEM/F-12, GlutaMAX™ supplement  500 mL10565018
KnockOut™ DMEM500 mL10829018
DMEM, low glucose, pyruvate, no glutamine, no phenol red  1 L                           11054001
DMEM/F-12500 mL11320033
DMEM/F-12, HEPES500 mL11330032
RPMI 1640 Medium500 mL11875093
RPMI 1640 Medium, no glucose500 mL11879020
DMEM, high glucose500 mL11965092
DMEM, high glucose, pyruvate500 mL11995065
IMDM500 mL12440053
Advanced DMEM/F-12500 mL12634010
RPMI 1640 Medium, HEPES500 mL22400097
Opti-MEM™ I Reduced Serum Medium500 mL31985070
MEM, no glutamine, no phenol red500 mL51200038
RPMI 1640 Medium (ATCC modification)500 mLA1049101
Cell Culture Supplements
MEM Non-Essential Amino Acids Solution (100X)                           100 mL                         11140050
Sodium Pyruvate (100 mM)100 mL11360070
B-27™ Supplement (50X), minus vitamin A10 mL12587010
N-2 Supplement (100X)5 mL17502048
B-27™ Supplement (50X), serum free10 mL17504044
L-Glutamine (200 mM)100 mL25030081
Insulin-Transferrin-Selenium-Ethanolamine (ITS -X) (100X)10 mL51500056
Glucose Solution50 mLA2494001
Cell Dissociation Reagents
Trypsin-EDTA (0.25%), phenol red       100 mL25200056
Trypsin-EDTA (0.05%), phenol red100 mL25300054
Cryopreservation Media
Recovery™ Cell Culture Freezing Medium50 mL    12648010

Serum
Fetal Bovine Serum, qualified, heat inactivated, USDA-approved regions500 mL                             10438026
Fetal Bovine Serum, qualified, United States500 mL26140079
Fetal Bovine Serum, qualified, One Shot™ format, United States500 mLA3160501
Serum-Free & Specialty Media
Neurobasal™-A Medium                       500 mL10888022
Neurobasal™ Medium500 mL21103049
Transfection Reagents
Lipofectamine™ 2000 Transfection Reagent1.5 mL     11668019

Gels, Fractionation Strips, & Membranes

Pre-Cast Protein Gels
NuPAGE™ 4-12% Bis-Tris Protein Gels, 1.5 mm, 10-well10 gels (1 box)          NP0335BOX
Bolt™ 4-12% Bis-Tris Plus Gels, 10-well10 gels (1 box)NW04120BOX
Novex™ 10% Tris-Glycine Mini Gels, WedgeWell™ format, 12-well10 gels (1 box)XP00102BOX

Kits and Assays

cDNA Synthesis, Library, & Cloning Kits

High-Capacity RNA-to-cDNA™ Kit                                      50 reactions           4387406
TaqMan™ Reverse Transcription Reagents200 reactionsN8080234

Lab Reagents and Chemicals

Flow Cytometer Buffers & Reagents

UltraComp eBeads™ Compensation Beads          100 tests  01-2222-42

General Buffers for Life Science

HEPES (1 M)                                            100 mL  15630080

Water

UltraPure™ DNase/RNase-Free Distilled Water  500 mL        10977015

Labeling & Detection Products

Enzyme Substrates

Pierce™ ECL Plus Western Blotting Substrate100 mL                       32132
SuperSignal™ West Femto Maximum Sensitivity Substrate100 mL34095

Oligos, Primers, Probes & Nucleotides

DNA Primers & Oligos

   Random Hexamers (50 μM)                   5 nmol   N8080127

Nucleotides

dNTP Set 100 mM Solutions                                    4 x 0.25 mL           R0181

Proteins, Enzymes, & Peptides

Enzyme Inhibitors

SUPERase• In™ RNase Inhibitor (20 U/μL)                2,500 units         AM2694
RNaseOUT™ Recombinant Ribonuclease Inhibitor5,000 units10777019

PCR & Cloning Enzymes

Phusion Hot Start II High-Fidelity PCR Master Mix100 reactions                F565S
DreamTaq PCR Master Mix (2X)200 reactionsK1071
Proteinase K, recombinant, PCR grade5 x 1.0 mLEO0492
SuperScript™ IV VILO™ Master Mix with ezDNase™ Enzyme50 reactions11766050
Platinum™ SuperFi™ Green PCR Master Mix100 reactions12359010
Platinum™ II Hot-Start PCR Master Mix (2X)200 reactions14000013
T4 DNA Ligase (1 U/μL)100 units15224017
DNase I, Amplification Grade100 units18068015
SuperScript™ IV Reverse Transcriptase10,000 units18090050

Standards, Ladders & Controls

DNA⁄RNA Ladders

    GeneRuler 100 bp Plus DNA Ladder, ready-to-use                    5 x 50 μg                SM0324
GeneRuler 1 kb Plus DNA Ladder, ready-to-use5 x 50 μgSM1333
E-Gel™ 1 Kb Plus Express DNA Ladder100 applications10488091

Protein Standards & Ladders

PageRuler™ Plus Prestained Protein Ladder, 10 to 250 kDa  2 x 250 μL                      26619
MagicMark™ XP Western Protein Standard250 μLLC5602

FAQs:

1 - How do I get set up to access the machines? 

  • Select the Request Access button (on one of the machines), complete the information requested and submit. You will receive an email message from Customer Service with your username and password within 48hours.

2 - To sign in without an access badge:

  • Select Access without a badge and enter your username and password.

3 - How do I Get an Access Badge?

  • For now, you can email Michael Nelson (min2016@med.cornell.edu) to pick up a badge OR you can request a badge at the machine itself.

4 - How do I Activate my Supply Center badge?

    • Sign in to lifetechnologies.com
    • Go to Supply Center Settings under Your Account
    • Enter your unique access badge number
    • You can now use your badge to access the Supply Center

5 - WhichPayment method should I use to make my purchases?

  • We strongly recommend using your current LOC for Life Technologies.
    • You can also have your badge directly linked to your Fund/WBS account number.
    • If you don’t have a LOC set up for Life Technologies or you anticipate using the Supply Center on a regular basis, you should set up a LOC for use with the Supply Center.
    • You can make modifications to your payment/cost center under Your Account at lifetechnologies.com

6 - Can I use my PCard with the Supply Center?

  • At this time, we are only able to link your LOC/Fund/WBS number to your access badge.

7 - How do I make a Purchase at the Supply Center?

Swipe your Access Badge to sign in or sign in without a badge by selecting Access without a Badge and enter your username and password.

  1. Confirm payment and cost center information; select Access (Note: you can make modifications to your payment/cost center values under Your Account at lifetechnologies.com
  2. The Supply Center door will unlock; open the door and remove the product(s) you need.
  3. Close the Supply Center door, select Done on the screen. An email order confirmation with details of your order will be sent to you.

8 - What if I selected an item in error?

  • If you’ve selected a product in error, please contact your SupplyCenter Administrator to begin the return process.

9 - What if the product I need to order is not at the Supply Center?

  • You will have to order that product through SciQuest

10 - What if there is not enough of the product I need to order?

  • You will have to order the total amount of the product you need through SciQuest.
  • You can make product stocking requests and contact your Account Representative directly from the Supply Center screen. These options are located as additional menu options under More…

 

What if I have further questions?

To find out more regarding product stock and make product requests please contact the Supply Center 800-955-6288 ext#46140 or email: supplycenters@lifetech.com

Purchasing questions please contact Anita Ferdinand 646-962-3691 aohallfe@med.cornell.edu

Life Technologies Representative please contact Smiti Khanna, Smiti.Khanna@thermofisher.com