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Human Immune Monitoring at Weill Cornell Medicine

Davis Cancer Immune Monitoring Core

Human Immune Monitoring activities at Weill Cornell are a collaborative effort between the Davis Cancer Immune Monitoring Core and Mass Cytometry Platform. A part of the Meyer Cancer Center, The Davis Cancer Immune Monitoring Core provides state-of-the-art technology platforms and top-notch services for single-cell, in situ, and multiomic analysis for cutting-edge immunology research and clinical studies to drive discoveries and improve treatments. 

 

Meyer Cancer Center

Open to All

The resources and services of the core facility are available for the study of cancer, as well as the human diseases beyond cancer.  Services are open to all investigators across multiple departments at Weill Cornell Medicine, Cornell University and Cornell-affiliated institutions. The facility also provides services to external investigators at both academic institutions and commercial enterprises. 

To learn more about Human Immune Monitoring at Weill Cornell Medicine, schedule equipment or request services, please visit our iLabs page.

Services

Spectral Flow Cytometry (Cytek Aurora)

Spectral Flow Cytometry (Cytek Aurora)

High-parametric cytometry enabled by the innovative 5-laser, 64-channel spectral cytometer Cytek Aurora, capable of 40+ colors using pre-optimized panels, or ~20-30 colors for routine experiments. It is available by reservation 24/7 to trained users.  

Resources and Publications 

Mass Cytometry

Mass Cytometry

Imaging Mass Cytometry (Fluidigm Hyperion)

The Fluidigm Hyperion imaging mass cytometry (IMC) system allows highly multiplexed tissue imaging, currently at up to ~40 markers per panel using metal-tagged antibodies and at 1 µm/pixel resolution for images comparable to light microscopy with minimal spillover between channels. This technology enables high-content, deep profiling of complex functional and signaling states of various cells in the tissue immune microenvironment (TIME) with spatial and structural context, unveiling the important interactions between these cells and their tissue and disease context.

CyTOF Mass Cytometry (Fluidigm Helios)

High parameter spatially resolved protein detection at sub-cellular resolution using mass cytometry time of flight in situ (CyTOF Hyperion).

Fluidigm IMC Workflow

Resources and Publications 

Mass Cytometry Protocols  

 

Education

Weill Cornell Medicine will sponsor and host a variety of informative and educational seminars focused on immune monitoring efforts throughout the year. Find upcoming center-wide events here, and keep an eye out for email announcements!

Recent Events

  • New England Cytek Aurora and Northern Lights User Group Meeting - Thursday, October 28, 2021 
  • Histowiz Special Seminar: "From Pixels to Cells: Segmentation Methods for Interrogating Single-Cell Data in Imaging Mass Cytometry Datasets"  - Tuesday, September 14, 2021  

Contact Information

Hiranmayi Ravichandran | hir4001@med.cornell.edu 

Hiranmayi Ravichandran

Amanda Pooter Hennemann | aph4002@med.cornell.edu 

Amanda Hennemann

Lab Hours

Spectral Flow Cytometry

Monday-Friday: 10 a.m.-6 p.m. by appointment only for assisted usage, training, consultation or 24/7 unassisted self-operation (training required) 

Mass Cytometry 

Monday-Friday: 10 a.m.-6 p.m. by appointment only

Lab Location