Human Therapeutic Organoid Core

The Weill Cornell Medicine Human Therapeutic Organoid Core (HTOC) facility serves as a central facility for human organoid technology to accelerate organoid-based scientific discovery. Organoids are mini-organs in a dish, made up of cells that self-organize and can more accurately recapitulate human biology. Human organoid technology has arisen as one of the best models to study human development and disease. The core facility will provide human pluripotent stem cell (hPSC) derived as well as tissue derived organoids as a fee-for-service. The central facility will overcome the technical challenges of quality control and reproducibility often found when using organoid technologies.  

The core facility will provide an opportunity for Weill Cornell Medicine laboratories to offset the high costs of establishing and sustaining this technology, in addition to the unnecessary duplication of resources. Organoids hold great promise to revolutionize healthcare with multimodal potential for new drug development, precision medicine, and regenerative medicine.  

Services

• hPSC culture reagent distribution at discounted prices   
• Consultation on the development and establishment of organoid systems 
• Hands-on training in organoid technology 
• Generation, banking, and distribution of normal human gut organoids derived from biopsy or autopsy tissues   
• Generation and distribution hPSC-derived pancreatic islet organoids (sc-islets) 
• Human organoid QC analyses: gene expression assessed via qRT-PCR; spatial protein localization assessed via immunofluorescence and immunohistochemistry (whole mount or cryosections); sc-islet differentiation efficiency assessed via stage specific immunostaining or flow cytometry  
• Custom project therapeutic organoid development   

Equipment

• Leica CM3050 S Cryostat  
• Applied Biosystems QuantStudio 5 Real-Time PCR System  
• Lonza 4D Nucleofector  
• NL5 swept field confocal microscope  
• RCM2 point scanning confocal microscope  
• Cytospin 4 Cytocentrifuge  
• EVOS M7000 Imaging System