The Weill Cornell Medicine Proteomics and Metabolomics Core Facility was established in 2016 and provides mass spectrometry-based analysis of proteins, peptides, metabolites, and other biochemical molecules to WCM users, as well as external users from outside academic institutions and commercial enterprises.

Core Technologies/Services

• Protein Identification
• Protein Quantitation
• Post-Translational Modification (PTM)
• Metabolomics
• Advanced and Custom Proteomics, including PTM analysis, quantitative protein interactions via AP-MS and/or proximity labeling, multiplexed protein abundance profiling
• Targeted quantitative LC-MS analysis of small molecules and peptides, including low- and high-throughput applications
• Custom proteomic method development and refinement
• Quantitative proteomics and phosphoproteomics
• Protein interaction studies using AP-MS and proximity labeling (BioID, APEX2)
• Multiplexed analysis using TMT, label-free, SILAC
• Data dependent (DDA) and data independent (DIA) proteomic analysis
• PTM mapping and discovery
• High- and low-throughput targeted analysis by MRM: metabolites; peptides; small molecules
• Data analysis: SEQUEST, MaxQuant, R, Perseus, DIA-NN, SCiLS Lab, MassHunter

Equipment

• Orbitrap Fusion Lumos
• TimsTOF Pro2
• Orbitrap Q Exactive
• Easy nLC 1200 nano flow UPLC
• Nano Elute nano flow UPLC
• Vanquish UPLC
• Agilent 1260 HPLC
• Thermo Scientific Orbitrap Fusion Tribrid
• Agilent RapidFire 365 High-throughput system
• Agilent 6495 Triple Quadrupole
• Benchbot plate handler, autosampler, and SPE
• UHPLC and Data Analysis Systems
• Agilent 1290 UHPLC System
• Thermo Scientific Easy nLC 1000 UHPLC.
• Omics Computing Proteome Destroyer PC

For more information, please visit our iLab website.