The Neuroanatomy Electron Microscopy Core provides training and services in experiments on brain tissue. These primarily include histology, pre-embedding light, electron microscopic immunocytochemical methods, and in situ hybridization techniques. The core also assists with the planning of anatomical experiments as well as data collection, analysis, and interpretation.
Core Technologies/Services
- Training in neuroanatomical methods
- Perfusion fixation of rodent brain
- Light microscopic immunocytochemistry in brain sections
- Single and dual labeling electron microscopy (EM) immunocytochemistry in brain sections
- In situ hybridization on brain sections
- General brain histology
- Quantitative analysis of light and EM specimens
Equipment
- Hitachi HT7800 transmission electron microscope
- Tecnai Biotwin transmission electron microscope
- 2 Leica Ultracut Ultramicrotomes
- 3 Leica vibratomes
- AO Sliding micotome
- HybEZ hybridization oven
- Nikon light microscopes and digital cameras
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