The Microbiome Core Lab is located at the Jill Roberts Institute for Research in Inflammatory Bowel Disease of Weill Cornell Medicine. Our core specializes in providing institutional cost-effective, high-throughput processing of biological microbiome samples.
Services
DNA Extraction
- Fecal Samples
- Skin Swabs (16S Only)
- Provide Pre-extracted DNA for Processing
16S Sequencing
DNA Is Extracted Utilizing Promega Maxwell RSC 48 Instrument
The Core Will Use The 16S Illumina Amplicon Protocol From Earth Microbiome Project
Up To 960 Barcoded Primers Are Available
515F-926R Primers Will Target V4 & V5 Regions
Library Pool Will Be Sequenced PE250 On The MiSeq
Metagenomic Sequencing
DNA Is Extracted Utilizing Promega Maxwell RSC 48 Instrument
The Core Will Use The Illumina Nextera XT Preparation Kit
We Provide Multiplexing Capacities Of Up To 384 Samples
Library Pool Will Be Sequenced PE150 On The HiSeq 2500
Bioinformatics Data Analysis
Resources
The core uses Promega Maxwell RSC 48 instrument along with the Promega MaxPrep Liquid Handler for high-throughput DNA extraction.
We use the Hamilton Liquid Handler to achieve high-throughput 16S/Metagenomic processing.
16S Sequencing will be done on the Illumina MiSeq, Paired-End 250
Metagenomic Sequencing will be done on the Illumina HiSeq 2500, Paired-End 150
Contact Information
Jorge Gandara
Operations Manager, WCM CLC Microbiome Core Facility
(646) 926-4774
microbiome@med.cornell.edu
More Information
For more information, please visit the Microbiome Core website.
Contact Information
Core Contact: Jorge Gandara
Email: microbiome@med.cornell.edu
Phone: (646) 962-4774