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Microbiome Core

The Microbiome Core Lab is located at the Jill Roberts Institute for Research in Inflammatory Bowel Disease of Weill Cornell Medicine. Our core specializes in providing institutional cost-effective, high-throughput processing of biological microbiome samples.

Services

DNA Extraction

  • Fecal Samples
  • Skin Swabs (16S Only)
  • Provide Pre-extracted DNA for Processing

16S Sequencing

  • DNA Is Extracted Utilizing Promega Maxwell RSC 48 Instrument

  • The Core Will Use The 16S Illumina Amplicon Protocol From Earth Microbiome Project

  • Up To 960 Barcoded Primers Are Available

  • 515F-926R Primers Will Target V4 & V5 Regions

  • Library Pool Will Be Sequenced PE250 On The MiSeq

Metagenomic Sequencing

  • DNA Is Extracted Utilizing Promega Maxwell RSC 48 Instrument

  • The Core Will Use The Illumina Nextera XT Preparation Kit

  • We Provide Multiplexing Capacities Of Up To 384 Samples

  • Library Pool Will Be Sequenced PE150 On The HiSeq 2500

Bioinformatics Data Analysis

Resources

The core uses Promega Maxwell RSC 48 instrument along with the Promega MaxPrep Liquid Handler for high-throughput DNA extraction. 

We use the Hamilton Liquid Handler to achieve high-throughput 16S/Metagenomic processing.

16S Sequencing will be done on the Illumina MiSeq, Paired-End 250

Metagenomic Sequencing will be done on the Illumina HiSeq 2500, Paired-End 150

Contact Information

Jorge Gandara
Operations Manager, WCM CLC Microbiome Core Facility
(646) 926-4774
microbiome@med.cornell.edu

More Information

Contact Information

Core Contact: Jorge Gandara

Email: microbiome@med.cornell.edu

Phone: (646) 962-4774