Summary
The WCM CLC Flow Cytometry Core Facility offers state-of-the-art fluorescence activated cell sorting (FACS) instruments and services, and expertise in their applications, to the Weill Cornell Medicine (WCM) community and to outside investigators. Services include support for cell counting, cell sorting, biomarker detection, and protein engineering projects. The facility also provides consultation on project design and data analysis, and offers educational workshops, seminars and training.
Services
The WCM CLC Flow Cytometry Core Facility provides full service (i.e., assisted use) for a wide variety of fluorescence activated cell sorting (FACS) applications, including sorting and analysis of cell surface markers, analysis of expression of intracellular proteins and separation of cell suspensions into sub-groups or single cells. Flow cytometry applications are optimized in close collaboration with investigators and with the staff of the CLC genomics and epigenomics, proteomics and metabolomics, and imaging core facilities, to achieve best possible materials for subsequent study. The Flow Core Facility also offers training in all aspects of flow cytometry, including hands-on use of sorters and analyzers. Soon to be implemented: investigators will be able to use selected instrumentation in the core facility 24/7 without staff assistance (i.e., unassisted use) after training and certification by core staff.
Resources
Cell sorters: BD Influx: (1) fully enclosed BSL-2 rated hood; (2) 355, 405, 445, 488, 561, and 640 laser lines; (3) 22 fluorescence detectors; (4) sorts cell sizes 1-50 microns; (5) sorts into tubes, plates, and custom collection vessels; (6) 6-way simultaneous sorting (tubes only); and (7) customizable fluidics to accommodate sensitive cells. BD FACSAria II SORP: (1) fully enclosed BSL-2 rated hood; (2) 355, 405, 488, 561, and 640 laser lines; (3) 18 fluorescence detectors; (4) sorts cell sizes 1-35 microns; (5) sorts into tubes, plates, and custom collection vessels; (6) 4-way simultaneous sorting; and (7) unassisted operation for trained core users.
Analytical cytometers: BD/Cytek FACSCalibur DxP 11: (1) 405, 488, 561, and 640 laser lines; (2) 11 fluorescence detectors; (3) analysis from single tubes, tube carousel, and 96-well plates; (4) unassisted operation for trained core users. BD FACScan: (1) 488 and 640 laser lines; (2) 4 fluorescence detectors; (3) analysis from single tubes; (4) unassisted operation for trained core users.
Data analysis: Site license for FlowJo analysis software.
More Information
Publications
Contact Us:
Jason McCormick
Co-Manager, WCM CLC Flow Cytometry Core Facility
(212) 746-3387
jam2073@med.cornell.edu
Tomas Baumgartner
Co-Manager, WCM CLC Flow Cytometry Core Facility
(646) 926-7611
tob2008@med.cornell.edu
For more information about services and pricing, to register as a user of the core, to submit samples, to schedule time on the instruments, and/or to arrange for training, please go to the WCM iLab portal at http://wcmc.corefacilities.org.