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Genomics


Summary

The Genomics Core Facility of the WCM Core Laboratories Center (CLC) provides state-of-the-art instruments and services, and expertise in their applications, to the Weill Cornell Medicine (WCM) community and to outside investigators.  Resources and services include next generation sequencing, single cell genomics, nucleic acid mass spectrometry, and real-time PCR.  The facility provides consultation on project design and data analysis, and offers seminars, educational workshops and training.

Overview

Location:  The core is located on the 1st and 4th floors of the “A” building and the 5th floor of the “C’ building at 1300 York Avenue, New York, NY.  

WCM Core Laboratories Center (CLC):  The WCM CLC was established in 2015.  The CLC includes core facilities that offer resources and services in genomics, proteomics and metabolomics, flow cytometry, imaging (e.g., optical and electron microscopy, high content screening, MRI, PET/CT, and ultrasound), synthetic and analytical chemistry, and nuclear magnetic resonance (NMR).

Open to all:  The resources and services of the core facility are open to all investigators at Weill Cornell Medicine, Cornell University and Cornell-affiliated institutions.  The facility also provides services to external investigators at both academic institutions and commercial enterprises.

Services

Next Generation Sequencing-

NGS genomics applications include exome capture, whole genome and targeted region de novo sequencing and resequencing, targeted region and whole transcriptome profiling, small RNA analysis, and metagenomics projects.

Single Cell Genomics

The core offers strong support for single cell genomics projects with a 10X Genomics Chromium system, a Drop-seq instrument, BioRad/Illumina ddSEQ Single-Cell Isolator, access to a Fluidigm C1 single-cell auto prep platform, and close collaboration with the CLC Flow Cytometry Core Facility.

Real-time PCR

An Applied Biosystem QuantStudio 6 Flex Real-Time PCR System (384-well and 96-well) uses a fluorescence detection method for quantitative and qualitative analysis of nucleic acid sequences including gene expression, regulation and variation. This system is compatible with SYBR and TaqMan assays as well as several other dye assays.  It can detect as small as a 1.5 fold change with 10 logs of linear dynamic range. 

Sanger Sequencing & Fragment Analysis

The core offers a sample “drop and ship” location and an express mail service to facilitate WCM investigators’ access to Sanger sequencing and fragment analysis services done on two ABI 3730xl DNA Analyzers in the Cornell Biotechnology Resource Center (BRC) Genomics Facility, located on the Cornell University campus in Ithaca, NY.  All orders must be placed online.  Once you have placed your order, you will receive an order number.  Sample preparation instructions are available online.  Send your samples in unpadded, labeled envelope.  Write your order number, name, telephone number, and lab location on the envelope.  Drop off the sealed, labeled envelope containing your samples in the DNA Sequencing Drop-Off Box, located in the basement corridor of the B building (near the mail room by the Federal Express drop box) at 1300 York Avenue, New York, NY.  Samples are sent overnight by Federal Express to the BRC Genomics Facility on the Cornell Ithaca campus, with shipments leaving New York every Monday through Thursday, at 4:30 pm (except on institutional holidays).  

Consultation

Seminars, workshops and training

Contact Information

Core Contact: Jenny Xiang, M.D.

Email: jzx2002@med.cornell.edu

Phone: (212) 746-4258

Lab Hours

Monday-Thursday

8:30 am - 4:30 pm

Friday: 8:30 am - 4:00 pm