Microscopy and Image Analysis
Core Facility

COS-7 cells expressing abnormal form of microtubule plus-end binding protein. MTs = red, yellow = plus-end binding protein

Credit: Katsuhiro Kita, Paraskevi Giannakakou (WCM)

Fresh mouse colon (unfixed, unstained) imaged using multiphoton microscopy. Green and Blue: autofluorescence at short and long wavelengths; red: Second Harmonic Generation (SHG)

Credit: Manu Jain, Sushmita Mukherjee (WCM)

Primary human adipocytes isolated from breast tissue were stained with lipidTox (red)

Credit: Priya Bharadwaj, Kristy Brown (WCM)

Gastric cancer cell stained with endogenous MT-end binding protein and MTs. MTs = green, red = end-binding protein

Credit: Katsuhiro Kita, Paraskevi Giannakakou (WCM)

Lipoproteins (red) being digested by macrophages (green)

Credit: Inna Grosheva, Frederick Maxfield (WCM)

Two living cells showing fluorescent sterol (green), a fluorescent lipid (blue) and an iron-carrying protein (transferrin, red)

The yellow color indicates places where cholesterol has entered an organelle into which the transferrin has been delivered.

Credit: Mingming Hao, Frederick Maxfield (WCM)

Lipoproteins (red) being digested by macrophages (green)

Credit: Inna Grosheva, Frederick Maxfield

A microglial cell (green) has internalized Alzheimer’s amyloid protein (red)

Credit: Amitabha Majumdar, Frederick Maxfield (WCM)

Gastric cancer cell treated with a tool compound showed a round and flat phenotype with strange MT bundles. MTs = green, purple = DAPI

Credit: Katsuhiro Kita, Paraskevi Giannakakou (WCM)

Circulating Tumor Cells (CTCs) pre-treatment with taxane

Credit: David Worroll, Paraskevi Giannakakou (WCM)

Circulating Tumor Cells (CTCs) post-treatment with taxane

Credit: David Worroll, Paraskevi Giannakakou (WCM)

NEW EQUIPMENT COMING IN EARLY 2024

(Click images for details)

Incucyte SX5

AxioObserver 7

EQUIPMENT ADDED IN 2023

(Click images for details)

AxioScan 7

AxioScope 5

EQUIPMENT ADDED MID-LATE 2022

(Click images for details)

Ultramicroscope II

Leica Stellaris 8

Acquifer HIVE

Additional advanced microscopy services
available through Weill Cornell Medicine’s institutional membership at the link below

New York Structural Biology Center (NYSBC)

The Imaging Core Facility strives to assist users with all their microscopic imaging needs, from the conception of an idea, to final image analysis and presentation. Our specific offerings are listed below.

Microscopy

Our Facility offers a variety of microscopes to our users, which are all available for independent, as well as limited assisted use. These include regular widefield microscopes for fluorescence and brightfield imaging, laser scanning microscopes (confocal and multiphoton), super-high resolution microscope (Zeiss Airyscan), automated high content optical microscopy, and electron microscopy.

Image analysis

We provide access to several image analysis software packages, and image analysis workstations that are optimized for handling large amounts of imaging data. The software we have current licence for include MetaMorph, MetaXpress and Imaris. In addition, we have Fiji/ImageJ on all our workstations.

Other services

In addition to microscopy and image analysis, our Core Facility also provides basic histology services. These include sectioning and H&E staining of tissues for light microscopy, and tissue processing for electron microscopy (including sample preparation for Scanning EM and Immuno-EM). In addition, our Core Facility oversees the use of a Phosphorimager and two X-ray film processors.

Education and Consultation

Our Education program consists of training users individually and in small groups. In addition, we offer free consultation of experiment design - including suggesting the best microscope for specific applications, optimal dyes to use, need for specialized imaging chambers etc. Consultation for image analysis are also provided.

Microscopy and Image Analysis Core Facility

NEW EQUIPMENT COMING IN EARLY 2024

(Click images for details)

EQUIPMENT ADDED IN 2023

(Click images for details)

EQUIPMENT ADDED MID-LATE 2022

(Click images for details)

Additional advanced microscopy servicesavailable through Weill Cornell Medicine’s institutional membership at the link below

Microscopy

Image analysis

Other services

Education and Consultation

Microscopy and Image Analysis
Core Facility

Additional advanced microscopy services
available through Weill Cornell Medicine’s institutional membership at the link below